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IF: 52.7 Xu Y, Zheng Y, Liu Y, et al. Ninjurin-1 mediates cell lysis and detrimental inflammation of PANoptosis during influenza A virus infection[J]. Signal Transduction and Targeted Therapy, 2025, 10(1): 1-13. | IF: 45.5 Chen Y, Chen S, Liu Z, et al. Red blood cells undergo lytic programmed cell death involving NLRP3[J]. Cell, 2025, 188(11): 3013-3029. e19. | IF: 52.7 Ma B, Ju A, Zhang S, et al. Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease[J]. Signal Transduction and Targeted Therapy, 2023, 8(1): 229. | IF: 26.6 Wu W, Pu Y, Gao S, et al. Bacterial Metabolism-Initiated Nanocatalytic Tumor Immunotherapy[J]. Nano-Micro Letters, 2022, 14(1): 1-21. | IF: 37.3 Zheng Z, Zeng X, Zhu Y, et al. CircPPAP2B controls metastasis of clear cell renal cell carcinoma via HNRNPC-dependent alternative splicing and targeting the miR-182- 5p/CYP1B1 axis[J]. Molecular Cancer, 2024, 23(1): 4. | IF: 30.9 Li C, Lu Y, Li Y, et al. Liver-breast communication of adipocyte-oriented exosomes drives primary mammary cancer progression[J]. Cell Metabolism, 2025. | IF: 18.9 Sun J, Yang F, Wang L, et al. Delivery of coenzyme Q10 loaded micelle targets mitochondrial ROS and enhances efficiency of mesenchymal stem cell therapy in intervertebral disc degeneration[J]. Bioactive Materials, 2023, 23: 247-260. | IF: 18.9 Wei X, Wang L, Duan C, et al. Cardiac patches made of brown adipose-derived stem cell sheets and conductive electrospun nanofibers restore infarcted heart for ischemic myocardial infarction[J]. Bioactive Materials, 2023, 27: 271-287. | IF: 16 Gao Y, Zhu Y, Wang H, et al. Lipid-mediated phase separation of AGO proteins on the ER controls nascent-peptide ubiquitination[J]. Molecular Cell, 2022, 82(7): 1313-1328. e8. | IF: 15.1 Chen X, Hao Y, Liu Y, et al. NAT10/ac4C/FOXP1 promotes malignant progression and facilitates immunosuppression by reprogramming glycolytic metabolism in cervical cancer[J]. Advanced Science, 2023, 10(32): 2302705. | IF: 12.8 Yang H H, Jiang H L, Tao J H, et al. Mitochondrial citrate accumulation drives alveolar epithelial cell necroptosis in lipopolysaccharide -induced acute lung injury[J]. Experimental & Molecular Medicine, 2022, 54(11): 2077-2091. |
Cas9X®海星生物开发的全新“微量化”慢病毒系统是专门为稳转细胞株设计的生产工艺，其核心就是将病毒包装过程“微量化”并与“稳转株构建”进行整合，全过程集成到一个6孔板内完成，从而实现整体的“微量化”，大幅提升了稳转细胞株的构建效率，帮助所有稳转株客户大幅节约经费。全新的“微量化系统”概念，将创造一种全新“低成本”、“高效率”的稳转株获得体验。
20种促销细胞株特价4980元（运输干冰费需加200元）
293T、HEK293、A549、HCT116、Hela、786-O、DU145、U251、PC-3、SiHa、B16、B16-F10、CHO、CHO-K1、DLD-1、TCCSUP、AGS、C-33A、Cal-27、HT-29
项目要求 | |
目的基因 | ORF≤3Kb（目的基因片段不大于3Kb） |
检测方式 | Q-PCR检测（mRNA表达水平检测） |
交付标准 | ≥2倍NC表达量（mRNA表达量是阴性对照组的2倍以上） |
交付内容 | 稳转细胞株2管，NC细胞株2管（2x105 cells/管） |
CMV启动子驱动目的基因表达；细胞株携带：puromycin抗性和绿色荧光（eGFP） | |
| 实验流程
| 实验案例
海星生物Haixing Biosciences基因编辑服务发表部分文章：
1. 影响因子为15.992
Li W, Ali T, Zheng C, et al. Anti-depressive-like behaviors of APN KO mice involve Trkb/BDNF signaling related neuroinflammatory changes[J]. Molecular Psychiatry, 2022, 27(2): 1047-1058
2. 影响因子为8.32
Li W, Ali T, Zheng C, et al. Fluoxetine regulates eEF2 activity (phosphorylation) via HDAC1 inhibitory mechanism in an LPS-induced mouse model of depression[J]. Journal of neuroinflammation, 2021, 18(1): 1-19.
3. 影响因子为5.59
Xiao P, Chen J, Zeng Q, et al. UNC5B Overexpression Alleviates Peripheral Neuropathic Pain by Stimulating Netrin-1-Dependent Autophagic Flux in Schwann Cells[J]. Molecular Neurobiology, 2022: 1-15.
咨询“微量化”稳转细胞株构建，请致电 400-990-0791 / 17712673685（微信同号）
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